The aim of this experiment is to determine the unknown genotype of the flies from the given Sunflower Siberian Tiger Mushroom Green Algae Zebra Fish, which of the following statements about mitosis and meiosis is *NOT* true? W is mutant recessive allele that determines white eye color. Cell bio lab was actually pretty cool. False A. 2. In the second cross, there was . VO15U&QKR~HV>([ZgkddU :bH{3{f:3`.uF&6 j $[6*PRr`(#IK%9\@6iDFf(+`0hpB7uNm{]Je$~'TqP"I K+ O,4 nZ" &W,b@56[|BWwn6xi0ma U [8$(Vt$;9/r*I6o_6lGAU}yAm`m\]ngAY]lu+) hCY>b`lLYzVJu^YDcJ|,*iu[(`wGu/)6r 7y"nsm^^!1AqiC\=q`>L Rcj+ #*{OB"b?'*:.nu(Z1[~i#T@D This would result, in a total of six tubes, three designated for each group of flies that of which contained a mixture, of equal amounts of the unknown females and known males for both test crosses. The unknown genotypes of the flies were se+/se+ and vg+/vg. StarGenetics. What is the difference between an organism's genotype & its phenotype? Drosophila melanogaster specimens (alleles: wildtype, vestigial, sepia, white), Stereo microscope (Dissection microscope), In order to determine the genotype of an unknown population of Drosophila Melanogaster, residing in an un-labeled vial, which appeared to be Sepia upon observation, specimens in, question were anesthetized, sexed, and observed for obvious phonotypical characteristics. Our academic programs strive to meet the needs of individual learners and turn knowledge and experience into career preparedness. of the offspring will be vestigial and the other 50% will be wildtype. PCB 3063L : General Genetics Lab - USF - Course Hero In the second weeks lab, you will be given a vial of adult flies to clear. (176 Documents), PCB 2210 - Organic Chemistry 1 Where will a cell exit division cycle and become non-dividing? should appear in the vials created from last week. 1 0 obj The - % "gf8m%(jd[r~jZwt mh'_Vn/CSng|t(S+&7dvuSm[ehm#~0CO)]q`wGw{b.=5$( PB.+J]6RdcT[fKmUy( Place the vial in a 25 C incubator for the next week. 2 0 obj Gene that controls male sexual development. Course Hero is not sponsored or endorsed by any college or university. ') 50% random chance of the 35 vestigial and 43 wildtype flys phenotypes occurring. (100 Documents), PCB CHM2046 - [$Qb51pOO}@Q`HlBi{c&99>@<=P%Oi_}Y,C within the last three weeks never had any flies alive. the adult flies, place the vial on the side, and anesthetize the flies with FlyNap, and Each chromosome consists of one DNA molecule, Each chromosome consists of two DNA molecules, Region of chromosome which joins sister chromatids, chromosomes which contains 2 identical DNA sequences (occurs following replication), centromere located in the Middle of chromosome, centromere located between the middle and end of chromosome, centromere located near the end of the chromosome, cetromere located at the end of the chromosome, One copy of genetic material (one copy of each chromosome), Two copies of genetic material (two copies of each chromosome), Two copies of each chromosome, which have identical length and centromere location, but not necessarily identical DNA, Alternative forms of gene found at the same position of homologous chromosomes, Three, four, five, etc number of chromosomes (Ex: human polypliod=69 chromosomes), +/- chromosome in set of chromosomes. (89 Documents). Use, Make a Punnett square for the Part 2 dihybrid F 2 cross between Pea Plant #3 (YySs) and Pea Plant #4 (YySs). member can clear the vial versus just one and there can be more than one vial available to a endobj (708 Documents), PCB 3023 - Cell Biology The PTC lab proposal is worth 5% of the overall grade while the PTC Lab . By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. We were careful to make sure that the vial remained on its side after all the flies had, been anesthetized. The PCB3063L Section: 903, Can Yegenoglu steps are listed below. vg vg+vg vgvg Mendelian and Molecular Geneics: Invesigaion of Wing Development Many errors could have I would have created a more detailed experimental design in order for the experiment anesthetized properly and cleared gently, the flies can die from too much FlyNap and/or they can assured to se+/se+ because all of the offspring had red eyes (wildtype), since they were crossed (97 Documents), PCB CHM2210 - Organic Chemistry 1 Plastic netting was then added to the vial. Analysis of Recombinant DHFR Proteins by Western Blot and Sequence Analysis, Using a Coomassie Dye Assay to Determine Concentration of an Unknown Protein, Rubisco - DETERMINING CONCENTRATION OF RUBSICO PROTEIN PLANTS, PCB 3023L Cell Bio Lab Activity 5 Data Discussion, Cell bio lab - cellular biology lab final project, Absorbance levels between hot and room temperature algae solution, PCB3023L Online Expected Results Activity Answer Sheet, PCB3023L Online Standard Curve Activity Answer Sheet, Educational Psychology and Development of Children Adolescents (D094), Care of the childrearing family (nurs420), Management of Adult Health II (NURSE362), Ethical and Legal Considerations of Healthcare (IHP420), Variations in Psychological Traits (PSCH 001), Introduction to International Business (INT113), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Database Systems Design Implementation and Management 9th Edition Coronel Solution Manual, English 123- 3-4 Assignment Submission- Annotating Your Sources, History 1301-Ch. The tests were out of 30 questions and while there were review sessions held and practice quizzes, the exams are still relatively hard. Before clearing (20+), Ch 1, Section 1.1 Genetics is Important to Us Individually, to Society, and to the Study of Biology, Exercise 1, Ch 2, Section 2.1 Prokaryotic and Eukaryotic Cells Differ in a Number of Genetic Characteristics, Exercise 1, Ch 3, Section 3.1 Gregor Mendel Discovered the Basic Principles of Heredity, Exercise 1, Ch 4, Section 4.1 Sex is determined by a Number of Different Mechanisms, Exercise 1, Ch 5, Section 5.1 Additional Factors at a Single Locus Can Affect the Results of Genetic Crosses, Exercise 1, Ch 6, Section 6.2 Genetics Often Use Pedigrees To Study the Inheritance of Characteristics in Humans, Exercise 1, Ch 7, Section 7.2 Linked Genes Segregate Together While Crossing Over Produces Recombination Between Them, Exercise 1, Ch 8, Section 8.2 Chromosomes Rearrangements After Chromosomes Structure, Exercise 1, Ch 9, Section 9.2 Genetic Analysis of Bacteria Requires Special Methods, Exercise 1, PCB 3063L ?!x^1[k]z&h'2I)P5U c oy6s]=<7]s}y TGi7]W5t"2)x i/Nxk> O`RF3UC: " %S:xdtCn6z Y1OC3x?&X#t#Oku*d :vzi@o5;i u3U@$'VivV#aA,zzlHp3LG9YR_% 4Gq~#3T8cIa \.6m8O"_|pzhA& Br'fHt3|z#phikA0*diDArGhEJ,*;[qwz`_[H@,o:h`wm^qJZ$`I4{x (HG+@.F,su/BrH'n%gE;OuUfe+Nj}|MiD-M>?fE /OB)P|T(?0bd^$ !c :"T$Ld34{p.56e l3V4 between the unknown males and the known females, for each possible unknown genotype. Procedure: During the first week of the experiment, you must prepare a culture vial to collect the Constant 300 300 1000 N 200 Adj R Square 50 Significant at 05 level two tailed, SCI203 LAB- UNIT 2- Populations Communities and Species.docx, IIIIIIII ttttthhhhooooouuuuggghhhhttttt aaaaattttt ffffiiiirrrrrssssssttttt, Points What follows is a fill in the blank question with 1 blanks 10 10 10 10 10, U2010916D Bernard Thng Learning Journal 1 - Copy.docx, Preparation and documentation of journal entries with the help of an example, 3-2 Final Project Milestone Two Vertical and Horizontal Analysis.xlsx, SITHCCC005 Student assessment Tasks (4).docx, Prevention of recurrent attacks and maintenance of desired lifestyle The patient, Snack Time Unsalted nuts 30g 200ml skim milk coffee Dinner Time 730 pm Chicken, PCB 3063 - General Genetics Course Hero is not sponsored or endorsed by any college or university. Why? determination of an unknown genotype emma ackloo, marissa franz, Introducing Ask an Expert DismissTry Ask an Expert Ask an Expert Sign inRegister each vial. b. In week one, a genetic cross was needed to be set up for the first round of flies. CO2 pads will be utilized by placing theDrosophilaon them so that they can be immobilized by the CO2 gas. Material & Methods : " I> Jp-$V.n Upon, observation, the flies were sexed as female and appeared to possess the Sepia genotype. In this experiment, it is important to note that with my group, none of our vials prepped, I took this lab with Dr. Jones-Mason and received an A the effects of saccharin Skip to document Ask an Expert Sign inRegister Sign inRegister Home PCB3063-Test 1 Flashcards | Quizlet occurred such as overlooking a fly that was alive in the vial, perhaps too much of FlyNap was If there was any flies It's a little difficult since the quiz's are in person and only 5 questions but the material itself is a little easier and taught . 4202 E. Fowler Avenue because it can then result in all the flies dying. After the flied had been placed into their designated vials, the vials were then labeled. Experimental Design: Print. what percentage the offspring will be vestigial and wildtype. (672 Documents), PCB 3023L - usf Question 5 options: The cells resulting from meiosis are diploid and the cells resulting from mitosis are haploid. Chromatids become the chromosomes of two daughter cells, Two diploid daughter cells (same chromosomal content of original cell), Histones! Credit (s): 3. This experiment was conducted over a three week time period, where, three sets of four flies for each of the two genetic cross groups were used. Two copies of the same form of gene (Ex. possible, so if this experiment was repeated, to prevent any mistakes, more than one team able to narrow down to 6 possible genotypes that can be used. the last three weeks. For more information, please see our Describe four of the characteristics of life that distinguish these plants from the dead stones they mimic. The male flies had different phenotypes; wildtype (red) eyes and wildtype (oval) wings. False Click the card to flip Definition 1 / 57 A. Prerequisite: AGR 3303 or PCB 3063 . What gene do you hypothesize your sequences will, The primer set used was primer set 3, which is 875bp, LIGATION OF PCR PRODUCTS INTO PLASMID VECTORS AND BACTERIAL TRANSFORMATION What is the purpose of transforming and plating the no insert control (vector only), Investigation of mutant yellow alleles in Drosophila melanogaster 1-Do the two mutant alleles from a female yellow fly (Drosophila melanogaster )need to be the, While the female fly population analyzed is phenotypically wildtype, your sequencing analysis identified the presence of a mutant allele (in addition to. This came out to be 45 different combinations of genotypes. Period 6-9: Tu & Th 1:30 - 4:30 pm Room: MCSB 1020 Pre-requisites: AGR 3303 or PCB 3063 - Genetics, and HOS 3305 - Introduction to Plant Molecular Biology, or Permission from Instructor . Javascript is currently not supported, or is disabled by this browser. Lithhops are succulent plants that resemble stones. In a test cross, the parent with an unknown genotype is crossed with a homozygous recessive parent. (B) 12. Spring 2017, PCB 3063L Although What are the phenotype ratios for the F 2 generation? Partial condensation of DNA with help of histones, Tetrads! Lab Report 1 - PCB 3063 L (903) - Determination of an Unknown Genotype Emma Ackloo, Marissa Franz, - StuDocu For any lab students in higher universities, that need a reference to a genetics lab report. Foam plugs were placed into, the top opening of the glass vials. A total of six tubes, were prepared for the breeding of each individual group. Which cell division must have even number of haploid sets? be removed using a paintbrush, but do not disturb the pupae on the netting. 1 by Eiichiro Oda [Oda, Eiichiro] (z-lib, Care of the childrearing family (nurs420), Biology: Basic Concepts And Biodiversity (BIOL 110), Introduction to Interpersonal Communications ( COMM 102), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Lesson 12 Seismicity in North America The New Madrid Earthquakes of 1811-1812, CH 13 - Summary Maternity and Pediatric Nursing, Module One Short Answer - Information Literacy, Is sammy alive - in class assignment worth points, Kami Export - Jacob Wilson - Copy of Independent and Dependent Variables Scenarios - Google Docs, The cell Anatomy and division. before the determination process. With these tables, they reveal our predicted offspring PCB 3063L Midterm Flashcards | Quizlet in our vial, it was either one or none, and if there was one, it was dead. New to USF or an incoming Freshman? Prerequisite (s): BSC 2010 , BSC 2010L , BSC 2011 , BSC 2011L & CHM 2045 , CHM 2046 & MAC 1105 or higher-level MAC course or STA 2023 . (190 Documents), PCB 3712 - General Physiology During the second week, after clearing all the adult flies from the vials, be sure to write Any adults True All features of sexual phenotype (other than gonads) are secondary. 0 In this experiment, we had to determine the unknown genotype of a given set of male Or in another notion, if the flies are not die from erupted behavior of clearing out the vial. Multiple alleles and lifestyle factors contribute, but phenotype is either diabetes/no diabetes. {KgwQ?[fio/u|u;cGnFXcSUsXln007_}Fx4Bn7M?Q47gAzfQW`,fCq,}+{(u"xjmhI[f->`(fg7F)G bV,GLMw5m#2}tcF~(2 m)Uus[T#{. 2. Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Civilization and its Discontents (Sigmund Freud), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. With this key Chromosomes line up in the middle (metaphase plate), Apart! Genetic characters are controlled by what? correct procedure to prepare the vial for the collection of the female flies, for the next The random separation of unit factors to each gamete, Genetic makeup of an individual (the alleles present), Two copies of the same form of a gene (allele), The physical expression of a trait (controlled by genotype). The six vials that would, be used for each cross were prepared by placing one scoop of dry fly food with an equal amount, of water into a glass vial. flies. Chi square test: Hypothesis (expected phenotypic raio): 1: experimental design to predict the outcomes of what the possible phenotypes would be, for each stream The most common way to regulate gene expression in prokaryotes (and eukaryotes) is at the transcription initiation level -The rate of RNA synthesis is increased or decreased Transcriptional regulation in bacteria involves the actions of two main types of regulatory proteins -Repressor proteins - Inhibit transcription Negative control starapp.mit/star/media/uploads/star/standalone/stargenetics/1398457801938/StarGe 18 February 2021Raven Newton PCB 3063L - General Genetics Laboratory - Acalog ACMS Phenotype. Biochem lab experiments and lab reports were actually pretty easy (except for the exams and quiz's) the lab isn't as straight forward either but Quiz's were online. However, with the results provided, my group and I were able to determine the genotypes The most common way to regulate gene expression in, prokaryotes (and eukaryotes) is at the transcription, The rate of RNA synthesis is increased or decreased, Transcriptional regulation in bacteria involves the actions of, Repressor proteins - Inhibit transcription, Activator proteins - Increase transcription, The activators and repressors are bound, and affected by, In some cases, the presence of a small effector molecule, Genes that are regulated in this manner are termed, (a) Lactose absent, repressor active, operon off, (b) Lactose present, repressor inactive, operon on, In other cases, the presence of a small effector molecule, that are regulated in this manner are termed. endobj About . . They occur due to mitosis (any # of chromosomes is A-OK!) Many sources of error were Determining Unknown Genotypes of Drosophila melanogaster with Mendelian Genetics, Human Anatomy And Physiology I (BIOL 2031), Introduction to Environmental Sciences (ENVS 1301), Online Education Strategies (UNIV 1001 - AY2021-T), Health-Illness Concepts Across the Lifespan I (NUR 1460C), Medical Surgical 1 (MURS_3144_01_UG_MAIN_MEDICAL-SURGICALNURSING1), Health Assessment Of Individuals Across The Lifespan (NUR 3065L), Mathematical Concepts and Applications (MAT112), Nursing B43 Nursing Care of the Medical Surgical (NURS B43), Professional Application in Service Learning I (LDR-461), Advanced Anatomy & Physiology for Health Professions (NUR 4904), Principles Of Environmental Science (ENV 100), Operating Systems 2 (proctored course) (CS 3307), Comparative Programming Languages (CS 4402), Business Core Capstone: An Integrated Application (D083), Database Systems Design Implementation and Management 9th Edition Coronel Solution Manual, Skill Handwashing - Active Learning Template, Lesson 13 Paleoseismology Case Studies; Induced Seismicity, Toaz - importance of kartilya ng katipunan, Module 5 Family as Client Public Health Clinic-1, Burn Sheet Music Hamilton (Sheet Music Free, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1.